Posters and poster guidelines
Thank you for considering to present your work as a poster at Phages 2022.
Digital poster preparation and submission
- Page size: Prepare your poster as you would normally do for printing. You can prepare your poster in sizes A1 or A0, as the page size is not important for digitally presented posters.
- Naming your poster files: Name your poster files as follows: <your surname>-Phg22-Poster.pdf | <your surname>-Phg22-Poster.png | <your surname>-Phg22-Poster.jpg, etc. For example, for David Jones, name your file as Jones-Phg22-Poster.pdf. DO NOT name your poster files as, e.g., Oxford-poster, Phage2022, Oxford-phage-poster. Such files will be automatically rejected.
- Poster submission and deadlines: All poster presenters, whether attending virtually or in-person, are required to submit a digital version of their poster. Submit your final poster as both PDF (<5MB) and JPG/PNG (<1MB) files via the link below no later than 31st August 2022 (we must have received your poster abstracts by 15th August). Late posters may not be included in the symposium programme. Please DO NOT send your poster (or abstract) files by email. Please ensure you send us the very final version of your poster (as well as your poster abstract), as once published, it cannot be replaced.
The poster PDF files, whether the presenter is attending virtually or in-person, will be made available via the secure conference documents page to the conference participants before the meeting.
- the participants will be able to ask questions via the Zoom chatbox during the mid-conference break each day; and/or
- the participants can post their questions on Twitter at any time using the meeting hashtag #PhgOx22, as well as the poster specific hashtag (given under each poster abstract) – do tag @PhageOxford in your tweets. Do include your Twitter handle in your poster, if you have one.
Hardcopy posters: If attending in-person, you may bring along a printed copy of your poster (maximum A1 size) to be displayed during the conference.
Any further information about the poster presentations at this digital meeting will be available in the future.
Before uploading your poster, you must make sure that you follow ALL of the instructions above!
(Presenters in Bold)
Accepted poster abstracts will be displayed below. If your abstract has been accepted for presentation but it does not appear in the list below, please let us know as soon as possible by emailing PhageOxford@gmail.com.
Genome Analysis and antibiofilm activity of a novel phage RDN8.1 against multi-drug resistant and extensively drug-resistant biofilm forming uropathogenic E. coli isolates
Naveen Chaudhary1, Balvinder Mohan1, Ravimohan S. Mavuduru2, Yashwant Kumar3 Neelam TanejaI*1
1Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research,Chandigarh,India
2Department of Urology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
3Central Research Institute, National Salmonella and Escherichia Centre, Kasauli, India
We aimed to study whole genome and antibiofilm activity of a novel RDN8.1 active against multi-drug- resistant (MDR) and extensively drug-resistant (XDR) biofilm-forming Uropathogenic E. coli isolates. We isolated novel phage RDN8.1, from community raw sewage water belonging to the order Caudovirales and the family Autographiviridae. It has a large burst size of 250 plaque-forming units per infected cell. The lytic ability of phage RDN8.1 was tested at MOIs 0.01, 0.1, and 1.0 in time-kill assays, and the best killing was achieved at MOI 1.0. The lytic activity started within 2 hours and was sustained for up to 18 hours. The complete genome of Escherichia phage RDN8.1 is 39.5 kb with a GC content of 51.6%, consisting of 49 ORFs, and all ORFs are present in the direct strand. RDN8.1 genome displays closest similarity (Blastn identity 86–92.97%) with four phages (Citrobacter phage CR44b, Escherichia virus Vec 13, Citrobacter phage SH3, and Enterococcus phage EFA-1).. The phage was able to inhibit biofilm formation with a clear disruption of the biofilm structure. Endolysin found in the phage RDN8.1 genome might have a role in the disruption of biofilms. The phage is stable over a wide range of temperatures and pH. We did not find any genes encoding markers of temperate bacteriophages such as integrase, recombinase, repressor, and excisionase. Therefore, the RDN8.1 phage is considered a virulent bacteriophage. We provide detailed genome and antibiofilm activity information of a novel lytic Escherichia phage RDN8.1 that has the potential for inclusion into phage cocktails being developed for the treatment of urinary tract infections (UTIs) caused by biofilm forming highly drug-resistant UPEC isolates.
The Use of Lytic Phage in the Treatment of Bacterial Urinary Tract Infections, and the Potential of Re-sensitising Bacteria to Antibiotics Using Phage Exposure
Libby A M Duignan1, Isik Kaya1, Craig Winstanley1, Rachel Floyd1 and Jo L Fothergill1, Chloe James2
1 Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Ronald Ross Building, 8 West Derby Street, Liverpool L69 7BE
2University of Salford, 43 Crescent, Salford M5 4WT
Antimicrobial resistance (AMR) is a major global health issue. Escherichia coli is a common cause of urinary tract infections (UTIs) and one potential alternative with growing traction is the use of lytic phages. E. coli isolated from patients with UTIs (151 isolates), were used to test the efficacy of 56 novel lytic phages isolated from sewage. The bacterial panel were genome sequenced and extensively tested for antimicrobial resistance. This revealed a range of ST types including ST131. Antibiotic resistance testing showed many of the isolates were multidrug resistant and carried many AMR genes.The 56 new phages were used in cross-infection studies against the clinical isolates and showed that some phages displayed a broad infection range, up to 72% of the bacterial isolates tested. A subset were sequenced and bioinformatic analysis of the phage genomes confirmed they are obligate lytic phages that do not harbour toxin or virulence factor genes. Screening assays identified the emergence of in vitro bacterial resistance against some of the phages. These evolved isolates were then tested for antibiotic resistance and complex dynamics were revealed. Phage exposure led to examples of both resensitisation and increased AMR. Analysis using the in vivo Galleria infection model, showed that E.coli strains evolved in the presence of phages were less virulent than prior to phage exposure. This study highlights phages with clinical potential against a wide range of isolates from UTIs including MDR E.coli, however the need to further understand the complex interplay between phage therapy, bacterial adaptation and AMR during infection before this synergy is used in a therapeutic approach for UTIs.
Transcriptomic landscape of therapeutic Kayvirus phage during infection of Staphylococcus aureus
Adéla Finstrlová1, Ivana Mašlaňová1, Bob G. Blasdel Reuter2, Jiří Doškař1, Friedrich Götz3 and Roman Pantůček1
1Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
2Vésale Bioscience, Vésale Pharma, Noville sur Mehaigne, Belgium
3Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine Tübingen, University of Tübingen, Tübingen, Germany
The treatment of infections caused by human and veterinary pathogen Staphylococcus aureus is becoming worldwide healthcare concern due to the increasing resistance to antibiotics. A promising alternative to currently used drugs is represented by lytic phages from genus Kayvirus, but their use is impeded by the lack of knowledge of phage-bacterium molecular interactions. Here, we performed RNA sequencing of two S. aureus strains infected with Kayvirus bacteriophage K to decipher the transcriptomics of the phage lytic life-cycle and the host response. We found that the temporal transcriptional profile of phage K was comparable in both strains except for a few loci. The RNA-Seq data also revealed presence of phage non-coding RNAs, which may play a role in the regulation of phage and host gene expression. The response of S. aureus to phage K infection resembles a general stress response and involves upregulation of nucleotide, amino acid and energy synthesis and transporter genes and the downregulation of host transcription factors. Our results clarify the global transcriptional interaction between phage and host, which will ensure safer usage of phage therapeutics and may also serve as a basis for development of new antibacterial strategies. Acknowledgments: Supported by the project National Institute of Virology and Bacteriology (Programme EXCELES, ID Project No. LX22NPO5103) – Funded by the European Union – Next Generation EU and grant NU22-05-00042 from the Ministry of Health of the Czech Republic.
Polyphasic survey of A. hydrophila phages and Genomic Characterization of a novel lytic phage, Aeromonad phage B614
Tracey Antaeus D. Gutierrez1, Reuel M. Bennett2,3 and Donna May D.C. Papa2,3
1The Graduate School
2Research Center for Natural and Applied Sciences
3Department of Biology, College of Science
University of Santo Tomas, España, Manila 1015 Philippines
Bacteriophages are considered vital in the microbial ecosystems for their role in bacterial mortality and evolution. Aside from their ecological importance, these viruses are considered as viable candidates for therapeutic applications in view of the emergence of antibiotic resistance. However, there are limited studies about phages in the country, which hinder further understanding of the nature of these viruses. This study employs a polyphasic approach in classifying bacteriophages by determining phenotypic characteristics, including the stability of phages in different environmental conditions (pH, temperature, and saline concentrations) and genotypic properties. Twenty-seven (27) previously isolated Aeromonas hydrophila phages were acquired from the UST-Bacteriophage Ecology, Aquaculture, Taxonomy, and Systematics (BEATS) Reference Collection, and were initially propagated using the host bacterium, A. hydrophila BIOTECH 10089. Physiological characterization of phages through stability testing and host range showed the phages isolated from sewage and polluted river systems exhibited the most stability and polyvalency in infection. Moreover, the diversity of the phages in the collection was established through these characteristics, resulting in 71% viable phages with varying characteristics. Initial analysis of g23 sequences, coupled with the phenotypic characteristics of the phages, suggested a highly unique set of phages isolated from four sample types (sewage, lake, river, and aquaculture pond) that infect A. hydrophila and high similarity in marker gene sequence. The results indicate a widespread distribution of related Aeromonad phages in the country, with phenotypic characteristics that cannot be limited to its environment. Finally, this paper also described the genome characteristics of phage B614, a novel lytic bacteriophage isolated from sewage water. Phage B614 may present a new species of Aeromonas phage under unclassified Biquartaviruses in the country, showing only an ANI of 84.22% to the closest Aeromonas phages in NCBI (Aeromonas phage ASFD-1). The results presented in this study may present evidence of the micro diversity of bacteriophages in local environments and their role in the abundance, control, and the diversity of bacterial communities.
Gel preparations containing phages (S. aureus) for specific local use
Ludmila Mateju, Sona Necadova, Marie Vavrova, Lenka Zakova, Marek Vronka, Lubos Jakub, and Juraj Vronka
AUMED a.s., Komoranska 326/63, 143 145 Praha 4 – Modrany, Czech Republic
AUMED deals with immunobiological preparations in general. The issue of the use of phages in human medicine is one of the main topics of the company. In this context, AUMED is contacted by three types of domestic specialized clinical workplaces: gynecological clinics, clinics that work with implants (breast, joint) and cardio surgical fields. The topic in surgical workplaces is the urgent need to eliminate primarily nosocomial bacterial infections, showing signs of resistance to antibiotics and possibly biofilm formation. Implant manufacturers have provided samples of materials for research on the suppression of biofilm formation using phages (on animal models). The gynecological topic is primarily focused on bacterial contamination, which in connection with childbirth poses a risk of infecting the newborn. The product also has potential use in preventive medicine, as an alternative to overuse of antibiotics. A hydrogel was developed for the mentioned three medical specialties, which, thanks to its balanced composition, preserves the viability of the contained phage particles and at the same time does not lose the physical properties of the gel. Bacteriophage subtype S 10/57 (host strain S. aureus 1137) specific for S. aureus was used as a model phage. A gel based on PEG 35,000 was developed as a carrier for this phage model. This system has been tested in animal models (pig) for safety and efficacy. Based on the results of the cumulative irritation index, the system was evaluated as a product with a negligible reaction (application period of 10 days). We have proven that the bacteriophage is long-term stable and effective in this gel base. In all three mentioned medical fields, we try to qualify the product for practical use as a medical device. Medicinal products containing phages are currently unregisterable in the Czech Republic due to the fact that the EMA has not yet published competent guidelines. AUMED is open for cooperation in mentioned activities.
Staphylococcus aureus prophage-accessory gene encoding immunity to myovirus by abortive infection
Ivana Maslanova1, Lucie Kuntova1, Adela Indrakova1, Radka Oborilova2, Hana Simeckova1, Pavol Bardy1,3, Marta Siborova2, Tibor Botka1, Zdenek Farka2, Jiri Doskar1, Roman Pantcek1
1Department of Experimental Biology, Faculty of Science, Masaryk University, 611 37, Brno, Czech Republic
2Central European Institute of Technology, Masaryk University, 625 00, Brno, Czech Republic
3Department of Chemistry, York Structural Biology Laboratory, University of York, York, UK
Prophages play an important role in the virulence, pathogenesis or host preference, as well as in horizontal gene transfer in staphylococci. On the other hand, broad-host-range lytic staphylococcal kayviruses lyse most Staphylococcus aureus strains. Lysogenic S. aureus strains become immune to infection by closely related phages, but interactions between temperate and lytic phages in staphylococci are not understood. Here, we present a novel resistance mechanism towards lytic phages of the genus Kayvirus, mediated by S. aureus prophage accessory gene. Based on the predicted structure of the prophage-encoded protein, we assume its transmembrane localization. We demonstrated that the mechanism of action does not prevent the infecting kayvirus from adsorbing onto the host cell and delivering its genome into the cell, but phage DNA replication is halted. Changes in the cell membrane polarity and permeability were observed, which lead to prophage-activated cell death. Furthermore, we describe a mechanism of overcoming this resistance in a spontaneous host-range Kayvirus mutant. We conclude that the defence mechanism belongs to a broader group of abortive infections, which is characterized by suicidal behaviour of infected cells, thus ensuring the survival of the host population that is unable to produce phage progeny. Since the majority of staphylococcal strains are lysogenic, our findings are relevant for development of phage therapy.This work was supported by the project National Institute of Virology and Bacteriology (Programme EXCELES, ID Project No. LX22NPO5103) funded by the European Union – Next Generation EU and grants (NU22-05-00042 and NU21J-05-00035) from the Ministry of Health of the Czech Republic.
Oral Prophylactic Administration of Phages Reduced Salmonella Proliferation and Dissemination in Mouse
Chutikarn Sukjoi1, Songphon Buddhasiri2, Arishabhas Tantibhadrasapa1, Panupon Monkolkarvin1, Songbo Li1,3, Preeda Phothaworn4, Janet Y. Nale5, Angela V. Lopez-Garcia6, Manal AbuOun6, Muna F. Anjum6, Danish J. Malik7, Edouard E. Galyov8, Martha R. Clokie8, Sunee Korbsrisate*4, and Parameth Thiennimitr*1,9
1Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand
2Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand
3Youjiang Medical University for Nationalities, Cheng Xiang Road No. 98, Baise, Guangxi, 533000, P.R. China
4Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand
5Department of Veterinary and Animal Science, Northern Faculty Scotland’s Rural College, United Kingdom
6Department of Bacteriology, Animal and Plant Health Agency, Weybridge, United Kingdom
7Department of Chemical Engineering, Loughborough University, Loughborough, United Kingdom
8Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom
9Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai, 50200, Thailand
Salmonella enterica serovar Typhimurium (STM) is the significant causative agent of foodborne acute non-typhoidal salmonellosis (NTS). The rising of multi-drug resistance (MDR) STM strains is alarming healthcare worldwide. The alternative method, including bacteriophage (phage), is one of the most promising ways against MDR STM. In our previous studies, we found that an oral administration of two Salmonella lytic phages (ST-W77 and SE-W109 are Myovirus and Siphovirus, respectively) significantly attenuated the severity of acute NTS in mice (the manuscript is in submission). This study demonstrated that phages ST-W77 and SE-W109 could be therapeutic agents for MDR STM. However, the prophylactic phage approach will offer the proper control for Salmonella spreading, especially in the food supply chain. Here, we aimed to investigate the preventive effect of ST-W77 and SE-W109 in the mouse. Our preliminary data showed that orally fed mice with 2 x 1011 PFU ST-W77 and SE-W109 daily for seven days significantly decreased Salmonella numbers in STM-infected mouse tissues compared to the untreated control. Phage-prophylactic mice conferred markedly lower Salmonella numbers in their gut (colon, cecal, colon, and cecum) and systemic tissue (spleen). These data supported that preventive giving of both phages reduced STM colonization in mouse gut and systemic dissemination. Moreover, we detected significant numbers of phages in fecal shedding during the oral prophylactic period, indicating phages’ availability in the mammalian gastrointestinal tract even without their bacterial host. In conclusion, phages ST-W77 and SE-W109 are effective anti-Salmonella agents orally given before the infection.