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Welcome to Exosomes 2016 Oxford
Exosomes are small extracellular vesicles released by cells under a variety of conditions, which can carry cargo, such as proteins, RNA and DNA. When exosomes are taken up by recipient cells they can have important effects on their phenotype. Indeed, it has emerged that exosomes are an important part of the dialogue that occurs between cells. Unsurprisingly, they have also been shown to be involved in the pathology of various conditions, including cardiovascular disease, immune deregulation and cancer. They also have the potential to serve as therapeutic targets and diagnostic biomarkers. The aims of this meeting are to explore the latest findings in the field of exosomes and how these findings can be applied to the understanding, diagnosis and treatment of disease.
The symposium agenda will consist of several short ‘rapid fire talks’ complemented by posters. Presentations by ‘poster-only display’ are also welcome. If you have any questions or would like to discuss your presentation proposal, please contact us on Exosomes@LPMHealthcare.com.
Session 1: Biogenesis and Characterisation
Engineering extracellular vesicles – towards biomedical applications
Combining Size Exclusion Chromatography (SEC) & Tunable Resistive Pulse Sensing (TRPS) technology for the characterization of extracellular vesicles
A drug-resistant form of the microenvironmental sensor mTORC1 controls endolysosomal trafficking and an exosome switch
1140: Refreshment break, posters and networking
Decorating the surface of extracellular vesicles
Exosomes are made in at least two distinct endolysosomal compartments and carry different cargos
Session 2: Functions in Radiation and Stress Response
Role of microvesicles/exosomes in non-targeted effects (NTE) of radiation: an in vivo study
1300: Dr Simone Moertl
AG Klinische Strahlenbiologie
Institut für Strahlenbiologie
Helmholtz Zentrum München
Exosomes as transmitters of the radiation response
1320: Lunch break, posters and networking
1420: Dr David Carter
Reader, Department of Biological and Medical Sciences
Oxford Brookes University
The role of extracellular vesicles in the intercellular response to stress
Session 3: Functions in Reproduction
The Role of Prostasomes in Human Embryo Implantation
Placental exosome and microvesicles support pregnancy-related immune adaptations
1520: Dr Rebecca Dragovic
Senior Research Associate
Nuffield Department of Obstetrics and Gynaecology
University of Oxford, Women’s Centre, John Radcliffe Hospital
Oxford, United Kingdom
Investigating the role of human follicular fluid extracellular vesicles in the ovarian follicle
1540: Refreshment break, posters and networking
Session 4: Roles in Disease
1610: Dr Alexander Kapustin
Research AssociateBHF Centre of Excellence
King’s College London
London, United Kingdom
Vascular Smooth Muscle Cell Calcification is Mediated by Regulated Exosome Secretion
Can salivary extracellular vesicles be used in early diagnosis of oral cancer and to monitor its progression?
Changes in exosome cargo under drug and microenvironmental selection-new biomarkers and therapy targets
1720: Discussion and Close
High-throughput, Purification-free, Multiplexed Profiling of Circulating miRNA for Discovery, Validation, and Diagnostics
Juan Hidalgo de-Quintana, M. Tackett, G. Doran, C. Rafferty, A. Windemuth, J. Tytell, D. Pregibon
Abcam plc, 330 Cambridge Science Park, Cambridge, CB4 0FL, UK
Abstract: We have developed the Multiplexed Circulating microRNA assay that allows the detection of up to 68 microRNA targets per sample. The assay combines particlebased multiplexing, using patented Firefly hydrogel particles, with single step RT-PCR signal. Thus, the Circulating microRNA assay leverages PCR sensitivity while eliminating the need for separate reverse transcription reactions and mitigating amplification biases introduced by target-specific qPCR. Furthermore, the ability to multiplex targets in each well eliminates the need to…
Characterization of microvesicles from human bronchial fibroblasts and their impact on the myofibroblastic differentiation during bronchial asthma*
Katarzyna Kmiotek1,2; Milena Paw1, Dawid Wnuk1, Ewa Zuba-Surma1, Marta Michalik1, Jarosław Czyż1
1 Jagiellonian University, Faculty of Biophysics, Biochemistry and Biotechnology, Department of Cell Biology, Krakow, Poland
2 Malopolska Centre of Biotechnology, Krakow, Poland
Abstract: Reports on the involvement of microvesicles (MVs) as the mediators of intercellular communication in the development of bronchial asthma prompted us to estimate their role in fibroblast-to-myofibroblast transition (FMT). It is a key process associated with the airway wall remodeling regulated by the multiple intercellular interactions and proinflammatory cytokines (include TGF-β1). Our recent findings show the involvement of connexin(Cx)43, a gap junctional protein, in the regulation of FMT in bronchial fibroblasts. Here, we focused for the first time on the identification and characterization of MVs derived from primary human bronchial fibroblasts (HBFs) isolated from asthmatic (AS) and healthy, non-asthmatic (NA) donors. Differences in transported cargo between AS MVs and NA MVs…
Host-parasite interactions: are vesicles from the Leishmania flagellum a source of virulence factors?
Laura Makin and Eva Gluenz
Sir William Dunn School of Pathology, South Parks Rd, Oxford, OX1 3RE, UK
Abstract:….My research investigates the possibility that during Leishmania differentiation flagellar membrane is shed in the form of vesicles which provide a virulence factor delivery mechanism. Differential centrifugation was used to isolate extracellular vesicles from differentiating parasite culture or a control promastigote parasite culture. Fluorescence imaging suggests that a sub-population of Leishmania extracellular vesicles is flagellar-derived. Mass spectrometric analysis was used to profile the protein content of isolated vesicles. Known virulence factors and known vesicle-associated proteins were enriched in the differentiating sample in addition to a large number of uncharacterised proteins…
RASSF1C oncogene promotes invasiveness in cancer cells through enhanced exosomes release
Maria Laura Tognoli, Nikola Vlahov and Eric O’ Neill
CRUK/MRC Oxford Institute for Radiation Oncology, Old Road Campus Research Building, ORCRB, Roosevelt Drive, Oxford OX3 7DQ, UK
Abstract: RASSF1 (RAS association domain family 1) is one of the most frequently epigenetically inactivated tumour suppressor genes in sporadic human malignancies. High promoter methylation has been shown to associate with poor prognosis in breast, lung and colon cancers amongst others, and is being increasingly described as an independent prognostic indicator of overall survival in cancer patients. Correspondingly, RASSF1A protein has roles in a variety of key biological processes that restrain the development of cancer: apoptosis, cell cycle regulation, mitosis, and microtubule dynamics. However, in tumours with RASSF1 promoter methylation an alternative isoform…
Gold Sponsor and Exhibitor
Izon Science designs, manufactures and sells precision instrumentation for nano- and micro-scale particle analysis. Using its technology, Tunable Resistive Pulse Sensing (TRPS), thousands of particles can be accurately analyzed for their size, concentration and charge. The technology is highly flexible and directly measures a wide range of biological and synthetic particles with individual particle resolution. Applications include the characterization of extracellular vesicles, viruses, cells and nanoparticles and microbubbles for drug delivery research. Izon’s instruments are in use in research institutes, universities, and pharmaceutical and biotechnology companies around the world.
The HansaBioMed range from Newmarket Scientific provides essential tools for researchers in the field of exosome research, including the ExoTEST platform, a proprietary immune-based solution for the capture, quantification and characterisation of exosomes from biological fluids, Lyophilized exosomes from specific culture supernatants, Exosome Antibodies and Exosome Isolation Reagents. Newmarket Scientific also provides a broad range of library prep reagents for specific Next Generation Sequencing applications and PCR enzyme supermixes.
Malvern Instruments: Malvern’s technology and expertise supports pharmaceutical and biopharmaceutical research and testing, for better characterization and control of proteins and other biopolymers in solution, as well as the analysis of biological carriers used in drug delivery. The data that Malvern’s instruments provide helps accelerate research and product development, and enhances and safeguards product quality.
The Malvern NanoSight range of instruments utilizes Nanoparticle Tracking Analysis (NTA) to characterize nanoparticles from 10nm -2000nm in solution. Applications include exosomes microvesicles, protein aggregates, vaccines and viruses. Each particle is individually but simultaneously analyzed by direct observation and measurement of diffusion events. This particle-by-particle methodology produces high resolution results for particle size distribution and concentration, while visual validation provides users with additional confidence in their data. Both particle size and concentration are measured, while a fluorescence mode provides differentiation of labelled or naturally fluorescing particles.
Protein charge, size, mass, molecular weight and polydispersity are key measurement parameters. Malvern’s Zetasizer instruments use dynamic and static light scattering to measure size, molecular weight and protein charge; Viscotek size exclusion chromatography (SEC) systems and detectors enable the rapid, reliable characterization of molecular weight, mass and structure; Sysmex FPIA-3000 dynamic imaging allows size and shape characterization of protein aggregates and subvisible particles; the Archimedes system uses resonant mass measurement to provide particle counting capability, as well as aggregate mass determination; and the Viscosizer TD draws on capillary electrophoresis technology to enable measurement of particle size and concentration as well as formulation viscosity.
Spanning a wide range of applications these systems are used to help identify and develop better drug candidates, improve productivity in biomanufacturing and understand protein function. This may include the characterization of antibodies and understanding protein changes in disease; measuring protein stability for formulation development; optimizing the separation of biological materials in bioprocessing; and assessing the biocomparability of protein therapeutics.