Posters

Oxford Symposia - 2nd Annual

OLIGO 2016 OXFORD:

Antisense & Therapeutic Nucleic Acids

06 April 2016, St Hilda's College, Oxford, UK

Twitter: @LPMHealthcare, #OligoOxford

Posters and guidelines

Guidelines for poster preparation

Please prepare your poster in A1 portrait format (59cm wide x 84cm long). Do not laminate your poster, or use heavy material to print your poster. Further information about poster sizes can be found on the following link:

http://tinyurl.com/y7bf

Posters larger than A1 will only be displayed subject to the availability of space.

Maximum capacity 10 A1 potrait posters

Please ensure you have appropriate permissions for the publication of your abstract from the original copyright holders. Should you wish your abstract not to be published, please notify us in writing at the time of abstract submission.

>>Where can I print my poster in Oxford?

Posters will be displayed for the full duration of the symposium.

Accepted posters

(Presenters in Bold)

If your abstract has been accepted for presentation but it does not appear in the list below, please let us know as soon as possible by email on Oligomer@LPMHealthcare.com.


Efficient delivery of antisense agents using endocytic back fusion

Paul D. R. Dyer1, Susan A. Shorter1, Alex S. Gollings1, Monique M. A. Patrick1, Mark Eccleston2, Simon C. W. Richardson1

1 IDS laboratory, Faculty of Engineering and Science, University of Greenwich, Kent, UK,
2 Oncolytika Ltd., 1 The Furs, Eli, Cambridge, UK

Ineffectual cytosolic delivery and vector toxicity contribute to the limited use of antisense oligonucleotides (ASOs) as therapeutics. Anthrax toxin (Atx) requires cytosolic access, attenuation is facile, and it has been used here as part of a novel ASO delivery system, inhibiting Syntaxin5 (Synt5) expression. The conjugation of ASOs was achieved via the in-frame fusion of the S. cerevisiae DNA binding protein (GAL4) to an attenuated truncation of Atx lethal factor (LFn). Microscopic examination revealed that Texas-Red labeled analogues of LFn-GAL4 could be readily visualized in the cytosol, distribution diminished over a 24h period peaking at 4h. This observation was supported by subcellular fractionation, where epitope labeled LFn-GAL4 was readily detected in a crude cytosolic fraction as described by a variety of cytosolic (LDH) integral membrane (LAMP1) and peripheral membrane (EEA1) immunological markers…


Functionalising DNA nanostructures with aptamers for molecular recognition and dynamic response

Marco S L Tang1, Maia Godonoga2, Andrew B Kinghorn1, Yee-Wai Cheung1, Jonathan Heddle3

and Julian A. Tanner1

School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong
2 RIKEN, Saitama, Japan
3 Malapolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland

DNA aptamers are useful tools for molecular recognition which can be simply integrated into DNA nanostructures for molecular recognition with various potential applications in therapeutics and diagnostics. Here, we present integration of aptamers specific for the malaria biomarker Plasmodium falciparum lactate dehydrogenase into DNA origami scaffolds. Integrated aptamers can capture target biomarker as evidenced by atomic force microscopy (AFM). The captured protein retains enzymatic activity and was observed dynamically using high-speed AFM…