(Capacity 35) – The training course is now full. Please check with us before registration. We are also running a waiting list. Please let us know if you are interested in joining this list.
Next-generation sequencing (NGS) has become a routine technology in just a few years and bears the promise of making personalised medicine a reality, and changing the pace of diagnostics and research in all fields of biological and medical sciences to the level never seen before. What NGS can achieve in a short span of time NGS has never been possible with the conventional sequencing methods.
The NGS technology itself is developing at a rapid pace with the introduction of new concepts, definitions and terminologies, new performance platforms and analytical tools, and workflow plans. All this makes it a considerable challenge for the newcomers. The current training course is designed to unravel the mysteries of this exciting technology for the new entrants and will provide an introduction to the basic methods, available platforms, applications and future directions.
Course Agenda and Topics:
The Training course will be limited to a maximum of 35 participants. Places will be alocated on a first-come, first-served basis. The course will run from 8.30am to 5.00pm on 31 March 2014 in the Vernon Harcourt Room at St Hilda’s College.
Workstations will be provided for hands-on training; each workstation will be shared by 2-3 participants.
Trainers: Dr Mark Lindsay (University of Bath, UK) and Dr Joerg Mages (Partek)
8.30-9.20: Registration (Vernon Harcourt Room, South Building)
9.20: Welcome and Housekeeping
9.30: Dr Mark Lindsay: Introduction to RNA sequencing
10.30: Coffee Break
11.00: Dr Joerg Mages: NGS analysis in Partek®Flow®
12.00: Dr Joerg Mages: NGS in Partek Genomics Suite: Hands-on training
12.30: Lunch break
13.30: Dr Joerg Mages: NGS in Partek Genomics Suite: Hands-on training
14.30: Dr Joerg Mages: Advanced RNA-seq analysis in Partek Genomics Suite: Hands-on training
16.00: Dr Joerg Mages: Advanced RNA-seq analysis in Partek Genomics Suite: Hands-on training
Dr Mark Lindsay will introduce various aspects of the NGS technology.
1. Overview on mRNA and long non-coding RNAs and the application of RNAseq
2. Introduction to RNA databases
3. RNA preparation and quality assessment
4. Overview of next generation sequencing platforms.
Dr Joerg Mages will offer a hands-on training course in sequence data analysis.
Dr Mages will cover a complete start-to-finish data analysis, including, sample information management, raw data quality assessment, alignment and trimming, post-alignment quality control, Principal Components Analysis, Quantification, Differential gene/transcript expression, SNP calling and more. Learn how you can turn your raw data into biology using advanced statistics and powerful visualizations. Hands-on exercises will be integrated throughout the lesson for you to practice the techniques discussed.
1. NGS analysis in Partek®Flow®: From Fastq files to differentially expressed genes. Using state-of the art NGS tools was never that easy (Live demo, 1 hour)
2. NGS in Partek Genomics Suite: BAM file import, visualizations, mRNA quantification and SNV detection (Hands-on training, 1.5 hours)
3. Advanced RNA-seq analysis in Partek Genomics Suite: Detecting and eliminating batch effects, advanced Statistics for differential expression, alternative splicing and Pathway analysis. (Hands-on training, 2 hours)