The workshops will be of non-commercial nature and will tackle common problems faced with genome editing tools. The schedule of these workshops will be announced in the near future.
Workshop-1 by Merck KGaA, 6th April, 17.50, The Edward Boyle AuditoriumTitle: Genome Editing beyond simple knockouts: considerations for successfully engineering defined SNPs and transgene integrations.
ABSTRACT: Deriving knockout cell lines and animals has become relatively straightforward compared with projects focused on modeling disease SNPs. A common roadblock is encountered when CRISPR-based SNP conversion works in one cell type, but not in another cell type which is critical for generating biologically relevant information. Furthermore, it has been observed that some donor DNA formats work at different rates among different cell types. This workshop will cover strategies to help you make decisions about experimental design in genome editing which maintain project momentum and derive meaningful biological data.
Workshop-2 by Merck KGaA 7th April, 11.50, The Edward Boyle AuditoriumTitle: How to successfully perform exploratory primary screens using CRISPR/Cas9 and RNAi technologies
ABSTRACT: The simplicity and low cost of programming CRISPR-Cas systems has created possibilities for high throughput, genome wide interrogation of gene function. For example, a drug treatment of cells can be characterized by knocking out tens of thousands of genes in parallel to identify pathways that lead to drug resistance. In this workshop, the key considerations for executing screens will be discussed covering the entire experimental flow from gRNA design, to cell treatments, to deep sequencing and statistical analysis.
- Workshop-3 by Dr Barry Rosen and Colleagues (Discovery Sciences, AstraZeneca, and the Wellcome Trust Sanger Centre)